Covalent immobilization of albumin on the surface of medical polyvinyl chloride modified by plasma-imersion ion implantation

Abstract

This work demonstrates the effect of plasma-immersion ion implantation on the adsorption activity of medical polyvinyl chloride for hemocontainers with respect to albumin protein. Polyvinyl chloride samples were treated with 20 keV ions at a dose of 1016 ion/cm2. Using the method of infrared Fourier spectroscopy of multiple attenuated total internal reflection, changes in the
molecular structure after exposure to ion treatment are analyzed. A series of physical experiments on the attachment and washing of bovine serum albumin on samples of untreated and plasma-treated polyvinyl chloride was performed. It has been shown that protein covalently binds to the surface of polyvinyl chloride modified by plasma-immersion ion implantation. On untreated polyvinyl chloride, the protein is adsorbed without establishing a covalent bond with the surface and is completely washed off by a buffer solution with an ionic detergent. According to the results of the authors’ previous works on improving the biocompatibility of polymeric materials modified by ion implantation, it is assumed that covalent binding of the protein in its native conformation to the
surface of the hemocontainer will contribute to preserving the integrity of blood cells in it.