Novel bacteria-producers of osmoprotective compounds isolated from salinized natural and technogenic ecosystems of Perm krai: physiology, genetics and biotechnological potential

Authors

  • L.N. Ananjina Institute of Ecology and Genetics of Microorganisms UB RAS
  • A.A. Gorbunov Institute of Technical Chemistry UB RAS
  • A.A. Pyankova Institute of Ecology and Genetics of Microorganisms UB RAS
  • E.A. Shestakova Institute of Ecology and Genetics of Microorganisms UB RAS
  • D.O. Nazarov Institute of Ecology and Genetics of Microorganisms UB RAS
  • D.O. Egorova Institute of Ecology and Genetics of Microorganisms UB RAS
  • A.O. Voronina Institute of Ecology and Genetics of Microorganisms UB RAS

DOI:

https://doi.org/10.7242/2658-705X/2020.4.7

Keywords:

halophilic and halotolerant bacteria, osmoprotectant solutes, ectoine

Abstract

Screening of halophilic and halotolerant bacteria isolated from biocenoses of the industrial development area of the Verkhnekamsk potassium-magnesium salt deposit (Perm krai) for the ability to synthesize a biotechnologically significant osmoprotector - ectoine was carried out. For the study, representatives of the genera Halomonas and Chromohalobacter of the Halomonadaceae family of the Gammaproteobacteria class, as well as strains of bacteria belonging to the genera Arthrobacter and Rhodococcus of the Actinobacteria class were selected from the working collection of microorganisms of the Laboratory of Molecular Microbiology and Biotechnology of the «Institute of Ecology and Genetics of Microorganisms (Russian Academy of Science, Ural Branch)». In the course of 'Н NMR spectroscopy studies, ectoine was shown to predominate in the cells of the Halomonadaceae family: its proportion, depending on the strain, was 64,0-78,1%. Structural and functional analysis of genes encoding ectoine synthesis enzymes was carried out in the genomes of bacteria of the genera Halomonas, Chromohalobacter, Cobetia, Kushneria, Halotalea and Salinicola of the Halomonadaceae family, deposited in the public database of the National Center for Biotechnological Information of the United States (NCBI). A protocol for amplification of the ect-genes in bacteria of the studied taxonomic groups was developed and tested.

Supporting Agencies
Исследование выполнено при финансовой поддержке РФФИ и Министерства образования и науки Пермского края, проект № 17-44-590178, и в рамках государственного задания, номер госрегистрации темы АААА-А19-119112290008-4.

Author Biographies

  • L.N. Ananjina, Institute of Ecology and Genetics of Microorganisms UB RAS

    кандидат биологических наук, научный сотрудник лаборатории молекулярной микробиологии и биотехнологии, Институт экологии и генетики микроорганизмов УрО РАН - филиал Пермского федерального исследовательского центра УрО РАН ("ИЭГМ УрО РАН")

  • A.A. Gorbunov, Institute of Technical Chemistry UB RAS

    кандидат химических наук, старший научный сотрудник лаборатории синтеза активных реагентов, Институт технической химии УрО РАН - филиал Пермского федерального исследовательского центра УрО РАН ("ИТХ УрО РАН")

  • A.A. Pyankova, Institute of Ecology and Genetics of Microorganisms UB RAS

    инженер лаборатории молекулярной микробиологии и биотехнологии, "ИЭГМ УрО РАН"

  • E.A. Shestakova, Institute of Ecology and Genetics of Microorganisms UB RAS
    инженер лаборатории молекулярной микробиологии и биотехнологии, ИЭГМ УрО РАН
  • D.O. Nazarov, Institute of Ecology and Genetics of Microorganisms UB RAS

    кандидат биологических наук, старший научный сотрудник лаборатории молекулярной микробиологии и биотехнологии, "ИЭГМ УрО РАН"

  • D.O. Egorova, Institute of Ecology and Genetics of Microorganisms UB RAS

    кандидат биологических наук, старший научный сотрудник лаборатории молекулярной микробиологии и биотехнологии, "ИЭГМ УрО РАН"

  • A.O. Voronina, Institute of Ecology and Genetics of Microorganisms UB RAS

    инженер лаборатории молекулярной микробиологии и биотехнологии, "ИЭГМ УрО РАН"

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Published

2021-01-12

Issue

No. 4 (2020)

Section

Research: theory and experiment

How to Cite

Ananjina, L. ., Gorbunov, A. ., Pyankova, A. ., Shestakova, E. ., Nazarov, D. ., Egorova, D. ., & Voronina, A. . (2021). Novel bacteria-producers of osmoprotective compounds isolated from salinized natural and technogenic ecosystems of Perm krai: physiology, genetics and biotechnological potential. Perm Federal Research Centre Journal, 4, 76-84. https://doi.org/10.7242/2658-705X/2020.4.7