The role of polyamines in the regulation of bacterial persistence

Authors

  • A.G. Tkachenko Institute of Ecology and Genetics of Microorganisms UB RAS
  • E.A. Khaova Institute of Ecology and Genetics of Microorganisms UB RAS
  • N.M. Kashevarova Institute of Ecology and Genetics of Microorganisms UB RAS
  • L.Yu. Nesterova Institute of Ecology and Genetics of Microorganisms UB RAS
  • A.V. Akhova Institute of Ecology and Genetics of Microorganisms UB RAS
  • R.Yu. Sidorov Institute of Ecology and Genetics of Microorganisms UB RAS
  • I.V. Tsyganov Institute of Ecology and Genetics of Microorganisms UB RAS

DOI:

https://doi.org/10.7242/2658-705X/2020.2.4

Keywords:

persistence, antibiotics, tolerance, polyamines, gene expression

Abstract

Using the complex of methods including reporter gene fusions, quantitative reverse transcriptase PCR and gene knockout mutations the participation of stress response genes ( rpoS, rmf, relA и spoТ ) in persister cell, formation was revealed in E. coli batch cultures during transition to stationary phase. The expression levels of these genes are positively modulated by polyamines (putrescine, spermidine, cadaverine) in a concentration-depended manner. So, effects of polyamines accumulated by stationary phase cells are targeted to the potentiation of the functions of cell global regulators implicated in stress response and persister cell formation. The data obtained in this work might be used for developing novel approaches targeted to the potentiation of traditional clinical antibiotics or elaboration of new antibacterials.

Supporting Agencies
Работа осуществлялась при финансовой поддержке Российского фонда фундаментальных исследований (проект № 16-44-590279) и выполнена в рамках государственного задания (Тема № 01201353249).

Author Biographies

  • A.G. Tkachenko, Institute of Ecology and Genetics of Microorganisms UB RAS
    доктор медицинских наук, профессор, заведующий лабораторией адаптации микроорганизмов, Институт экологии и генетики микроорганизмов УрО РАН - филиал Пермского федерального исследовательского центра УрО РАН (ИЭГМ УрО РАН)
  • E.A. Khaova, Institute of Ecology and Genetics of Microorganisms UB RAS
    аспирант, ИЭГМ УрО РАН
  • N.M. Kashevarova, Institute of Ecology and Genetics of Microorganisms UB RAS
    младший научный сотрудник, ИЭГМ УрО РАН
  • L.Yu. Nesterova, Institute of Ecology and Genetics of Microorganisms UB RAS
    кандидат биологических наук, старший научный сотрудник, ИЭГМ УрО РАН
  • A.V. Akhova, Institute of Ecology and Genetics of Microorganisms UB RAS
    кандидат биологических наук, научный сотрудник, ИЭГМ УрО РАН
  • R.Yu. Sidorov, Institute of Ecology and Genetics of Microorganisms UB RAS
    аспирант, ИЭГМ УрО РАН
  • I.V. Tsyganov, Institute of Ecology and Genetics of Microorganisms UB RAS
    аспирант, ИЭГМ УрО РАН

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Published

2020-07-22

Issue

No. 2 (2020)

Section

Research: theory and experiment

How to Cite

Tkachenko, A. ., Khaova, E. ., Kashevarova, N. ., Nesterova, L. ., Akhova, A. ., Sidorov, R. ., & Tsyganov, I. . (2020). The role of polyamines in the regulation of bacterial persistence. Perm Federal Research Centre Journal, 2, 36-47. https://doi.org/10.7242/2658-705X/2020.2.4