Stability and functional properties of DNA-aptamers immobilized on the surface of carbon nanoparticles applicable in diagnostic and target therapy systems

Authors

  • M.B. Raev Institute of Ecology and Genetics of Microorganisms UB RAS
  • P.V. Khramtsov Institute of Ecology and Genetics of Microorganisms UB RAS
  • M.D. Kropaneva Institute of Ecology and Genetics of Microorganisms UB RAS
  • V.P. Timganova Institute of Ecology and Genetics of Microorganisms UB RAS
  • M.S. Bochkova Institute of Ecology and Genetics of Microorganisms UB RAS
  • S.A. Zamorina Institute of Ecology and Genetics of Microorganisms UB RAS

DOI:

https://doi.org/10.7242/2658-705X/2020.1.5

Keywords:

aptamers, DNA, RNA, carbon nanoparticles, diagnostics, nucleases, stability

Abstract

The technology has been developed for the preparation of conjugates of carbon nanoparticles with DNA aptamers. The synthesis and preservation of the structural and functional properties of the resulting structures from the standpoint of their stability during long-term storage have been studied and optimised. This concerns, first of all, the prospects of using such conjugates in in vitro diagnostics and targeted therapy. Of particular importance are the data on resistance to environmental influences and attack by biologically active compounds (endonucleases). It is the activity of these molecules to nucleic acids and their fragments that constitutes the main obstacle to the use of aptamers in in vivo diagnostics and therapeutic practice. The results obtained are in favour of a real solution to existing problems.

Author Biographies

  • M.B. Raev, Institute of Ecology and Genetics of Microorganisms UB RAS
    доктор биологических наук, ведущий научный сотрудник лаборатории экологической иммунологии, Институт экологии и генетики микроорганизмов УрО РАН - филиал Пермского федерального исследовательского центра УрО РАН (ИЭГМ УрО РАН); профессор кафедры микробиологии и иммунологии биологического факультета, Пермский государственный национальный исследовательский университет (ПГНИУ)
  • P.V. Khramtsov, Institute of Ecology and Genetics of Microorganisms UB RAS
    кандидат биологических наук, младший научный сотрудник лаборатории экологической иммунологии, ИЭГМ УрО РАН; ассистент кафедры микробиологии и иммунологии биологического факультета, ПГНИУ
  • M.D. Kropaneva, Institute of Ecology and Genetics of Microorganisms UB RAS
    инженер, ИЭГМ УрО РАН
  • V.P. Timganova, Institute of Ecology and Genetics of Microorganisms UB RAS
    кандидат биологических наук, младший научный сотрудник лаборатории экологической иммунологии, ИЭГМ УрО РАН
  • M.S. Bochkova, Institute of Ecology and Genetics of Microorganisms UB RAS
    кандидат биологических наук, научный сотрудник лаборатории экологической иммунологии, ИЭГМ УрО РАН
  • S.A. Zamorina, Institute of Ecology and Genetics of Microorganisms UB RAS
    доктор биологических наук, ведущий научный сотрудник лаборатории экологической иммунологии, ИЭГМ УрО РАН; профессор кафедры микробиологии и иммунологии биологического факультета, ПГНИУ

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Published

2020-04-17

Issue

No. 1 (2020)

Section

Research: theory and experiment

How to Cite

Raev, M. ., Khramtsov, P. ., Kropaneva, M. ., Timganova, V. ., Bochkova, M. ., & Zamorina, S. . (2020). Stability and functional properties of DNA-aptamers immobilized on the surface of carbon nanoparticles applicable in diagnostic and target therapy systems. Perm Federal Research Centre Journal, 1, 45-52. https://doi.org/10.7242/2658-705X/2020.1.5