Pregnancy-associated proteins and their role in the regulation of t-immune memory cell differentation

Authors

  • S.A. Zamorina Institute of Ecology and Genetics of Microorganisms UB RAS
  • V.P. Timganova Institute of Ecology and Genetics of Microorganisms UB RAS
  • M.S. Bochkova Institute of Ecology and Genetics of Microorganisms UB RAS
  • L.S. Litvinova Immanuel Kant Baltic Federal University
  • K.A. Yurova Immanuel Kant Baltic Federal University
  • O.G. Khaziahmatova Immanuel Kant Baltic Federal University
  • P.V. Khramtsov Institute of Ecology and Genetics of Microorganisms UB RAS; Immanuel Kant Baltic Federal University
  • S.V. Kochurova Institute of Ecology and Genetics of Microorganisms UB RAS
  • M.B. Rayev Institute of Ecology and Genetics of Microorganisms UB RAS

DOI:

https://doi.org/10.7242/2658-705X/2020.1.3

Keywords:

alpha-fetoprotein, chorionic gonadotropin, trophoblastic b-1-glycoprotein, T-cells of immune memory, pregnancy, CD45

Abstract

The role of chorionic gonadotropin (CG), alpha-fetoprotein (AFP) and pregnancy-specific β-1-glycoprotein (PSG) in the regulation of the processes determining the differentiation and maturation of naive T-cells into memory T-cells has been studied. It has been established that CG, AFP and PSG have a predominantly suppressing effect on the expression of CD28 and CD25 activation markers by naive T-cells (CD45RA+) and memory T-cells (CD45R0+), virtually without affecting the expression of CD71 proliferation marker. It has been established that the differentiation processes of naive T-cells and memory T-cells, under the influence of CG, AFP and PSG, are carried out with changing the expression of the - U2af1l4, Gfi1 and hTERT genes, which regulate the alternative splicing of the Ptprc gene (encoding CD45 molecule). At the same time, CG, AFP and PSG stimulated autocrine production of IL-2 with both naive and memory T-cells. Thus, for the first time, the modulating effects of the above-mentioned fetoplacental proteins in relation to the differentiation and functional activity of immune memory T-cells are shown.

Supporting Agencies
Работа выполнена при финансовой поддержке РФФИ, проект 16-44-590049, программы повышения конкурентоспособности (5-100) и субсидии «Организация проведения научных исследований 20.4986.2017/ВУ» БФУ им. И. Канта.

Author Biographies

  • S.A. Zamorina, Institute of Ecology and Genetics of Microorganisms UB RAS
    доктор биологических наук, ведущий научный сотрудник лаборатории экологической иммунологии, Институт экологии и генетики микроорганизмов УрО РАН - филиал Пермского федерального исследовательского центра УрО РАН (ИЭГМ УрО РАН)
  • V.P. Timganova, Institute of Ecology and Genetics of Microorganisms UB RAS
    кандидат биологических наук, младший научный сотрудник лаборатории экологической иммунологии, ИЭГМ УрО РАН
  • M.S. Bochkova, Institute of Ecology and Genetics of Microorganisms UB RAS
    кандидат биологических наук, научный сотрудник лаборатории экологической иммунологии ИЭГМ УрО РАН
  • L.S. Litvinova, Immanuel Kant Baltic Federal University
    доктор медицинских наук, заведующая базовой лабораторией иммунологии и клеточных биотехнологий, Балтийский федеральный университет имени Иммаиуила Канта (БФУ им. И. Канта)
  • O.G. Khaziahmatova, Immanuel Kant Baltic Federal University
    кандидат биологических наук, научный сотрудник лаборатории иммунологии и клеточных биотехнологий, БФУ им. И. Канта
  • P.V. Khramtsov, Institute of Ecology and Genetics of Microorganisms UB RAS; Immanuel Kant Baltic Federal University
    кандидат биологических наук, младший научный сотрудник лаборатории экологической иммунологии, ИЭГМ УрО РАН; научный сотрудник, БФУ им. И. Канта
  • S.V. Kochurova, Institute of Ecology and Genetics of Microorganisms UB RAS
    инженер лаборатории экологической иммунологии, ИЭГМ УрО РАН
  • M.B. Rayev, Institute of Ecology and Genetics of Microorganisms UB RAS
    доктор биологических наук, ведущий научный сотрудник лаборатории экологической иммунологии, ИЭГМ УрО РАН

References

  1. Zamorina S.A., Litvinova L.S., Urova K.A., Haziahmatova O.G., Timganova V.P., Bockova M.S., Hramcov P.V., Raev M.B., Ceresnev V.A. Rol’ al’fa-fetoproteina v regulacii proliferacii i funkcional’noj aktivnosti naivnyh T-kletok i T-kletok immunnoj pamati // Bulleten’ eksperimental’noj biologii i mediciny. - 2019. - T. 167. - No 4. - S. 454-458.
  2. Kudravcev I.V. T-kletki pamati: osnovnye populacii i stadii diferencirovki // Rossijskij immunologiceskij zurnal. - 2014. - 8(4). - P. 947-964.
  3. Kudrasova A.V., Kadyrova L.V. Differencirovka CD8+ kletok pamati pri beremennosti // Rossijskij immunologiceskij zurnal. - 2014. - T. 8(17). - No 1. - S. 79-82.
  4. Litvinova L.S., Mazunin I.O., Gucol AA., Sohonevic N.A., Haziahmatova O.G., Kofanova KA. Dozozavisimye effekty steroidnyh gormonov na ekspressiu genov GF11 i U2AF1L4 v T-limfocitah raznoj stepeni differencirovki // Molekularnaa biologia. - 2013. - T. 47. - No 4. - 656 s.
  5. Misarina L.V., Sotnikova N.U., Kudrasova A.V., Vasin I.A., Borzova N.U. Fenomen immunologiceskoj pamati pri beremennosti // Rossijskij immunologiceskij zurnal. - 2017. - T. 11(20). - No 3. - S. 425-426.
  6. Posiseeva L.V., Nazarov S.B., Tatarinov U.S. Trofoblast-specificeskij beta-glikoprotein v akuserstve i ginekologii. - Ivanovo: OAO <>, 2004. - 240 s.
  7. Raev M.B. Litvinova L.S., Urova K.A., Haziahmatova O.G., Timganova V.P., BockovaM.S., Hramcov P.V., Zamorina S.A. Rol’ trofoblasticeskogo pi-glikoproteina v regulacii molekularnogeneticeskih mehanizmov differencirvki T-kletok immunnoj pamati // Medicinskaa immunologia. - 2019. - T. 21. - No 1. - S. 49-58.
  8. Raev M.B. Sposob vydelenia i ocistki trofoblasticeskogo p-1-glikoproteina. Patent RF No 2367449 ot 20.09.2009g.
  9. Todosenko N.M., Urova K.A., Haziahmatova O.G., Litvinova L.S. Vlianie metilprednizolona na pozdnie etapy differencirovki CD4+ T-kletok pamati pri revmatoidnom artrite issledovanie in vitro // Fiziologia celoveka. - 2019. - T. 45. - No 3. - S. 92-98.
  10. Ceresnev V.A., Rodionov S.U., Cerkasov V.A., Malutina N.N., Orlov O.A. Al’fa-fetoprotein. - Ekaterinburg: UrO RAN, Izdatel’sko-poligraficeskij kompleks <>, 2004. - 376 s.
  11. Urova K.A., Sohonevic N.A., Haziahmatova O.G., Litvinova L.S. Citokinoposredovannaa regulacia ekspressii genov Gfil i U2afll4 aktivirovannymi T-kletkami s raznym differencirovocnym statusom in vitro // Biomedicinskaa himia. - 2016. - T. 62. - No 2. - S. 180-186.
  12. Butte J.M., Lee J.S., Jesneck J. CD28 costimulation regulates genome-wide effects on alternative splicing // PLOS One. - 2012. - Vol. 7. - No 6. e40032. DOI
  13. Dong M. [et al.] The effect of trophoblasts on T lymphocytes: possible regulatory effector molecules-a proteomic analysis // Cell Physiol. Biochem. - 2008. - Vol. 21. - No 5-6. - P. 463-472.
  14. Heyd F., ten Dam G., Moroy T. Auxiliary splice factor U2AF26 and transcription factor Gfi1 cooperate directly in regulating CD45 alternative splicing // Nat. Immunol. - 2006. - Vol. 7. - No 8. - P. 859-867.
  15. Holmes N. CD45: all is not yet crystal clear // Immunology. - 2006. - Vol. 117(2). - P.145-155.
  16. Kieffer T.E., Faas M.M., Scherjon S.A., Prins J.R. Pregnancy persistently affects memory T cell populations // J. Reprod. Immunol. - 2016. - Vol. 119. - P. 1-8.
  17. McNeill L., Salmond R.J., Cooper J.C., Carret C.K., Cassady-Cain R.L., Roche-Molina M., Tandon P., Holmes N., Alexander D.R. The differential regulation of Lck kinase phosphorylation sites by CD45 is critical for T cell receptor signaling responses // Immunity. - 2007. - Vol. 27. - No 3. - P. 425-437.

Downloads

Published

2020-04-17

Issue

No. 1 (2020)

Section

Research: theory and experiment

How to Cite

Zamorina, S. ., Timganova, V. ., Bochkova, M. ., Litvinova, L. ., Yurova, K. ., Khaziahmatova, O. ., Khramtsov, P. ., Kochurova, S. ., & Rayev, M. . (2020). Pregnancy-associated proteins and their role in the regulation of t-immune memory cell differentation. Perm Federal Research Centre Journal, 1, 31-37. https://doi.org/10.7242/2658-705X/2020.1.3