The role of thiol redox systems under a combined action of extreme temperatures and antibiotics in Escherichia coli

Authors

  • G.V. Smirnova Institute of Ecology and Genetics of Microorganisms UB RAS
  • E.V. Lepekhina Institute of Ecology and Genetics of Microorganisms UB RAS; Perm National Research Polytechnic University
  • N.G. Muzyka Institute of Ecology and Genetics of Microorganisms UB RAS
  • Z.Y. Samoilova Institute of Ecology and Genetics of Microorganisms UB RAS
  • A.V. Tyulenev Institute of Ecology and Genetics of Microorganisms UB RAS
  • K.V. Bezmaternykh Institute of Ecology and Genetics of Microorganisms UB RAS
  • M.A. Peters Institute of Ecology and Genetics of Microorganisms UB RAS
  • V.Y. Ushakov Institute of Ecology and Genetics of Microorganisms UB RAS; Perm State National Research University
  • M.N. Brysova Institute of Ecology and Genetics of Microorganisms UB RAS

DOI:

Keywords:

thiol redox systems, ciprofloxacin, ampicillin, temperature, bacteria

Abstract

Stresses induced by abrupt temperature shifts, antibiotics of different classes and by a combined action of nonoptimal growth temperatures and antibiotics are studied in bacteria Escherichia coli deficient by components of redox systems dependent on glutathione ( gshA, gor, grxA, grxB ) and thioredoxin ( trxA , trxB ). It is shown that the mutations under consideration significantly change the number of S-S bindings in proteins, the levels of oxidants (H2O2), reductants (glutathione) and the antioxidant gene expression, as well as the susceptibility of bacteria to antibiotics and extreme temperatures. The direction of influence (an increase or decrease in susceptibility) depends on the type of mutation and differs for antibiotics of different classes. Significant inverse correlations were observed between lgCFU (colony-forming units) and specific growth rate prior to antibiotic addition under all the situations investigated: the effect of mutations on the antibiotic activity at an optimal growth temperature, the effect of combined stresses and the effect of supplements that change the redox state.

Supporting Agencies
Работа выполнена при финансовой поддержке РФФИ (грант № 13-04-96039).

Author Biographies

  • G.V. Smirnova, Institute of Ecology and Genetics of Microorganisms UB RAS
    доктор биологических наук, ведущий научный сотрудник лаборатории физиологии и генетики микроорганизмов
  • E.V. Lepekhina, Institute of Ecology and Genetics of Microorganisms UB RAS; Perm National Research Polytechnic University
    кандидат биологических наук, инженер лаборатории физиологии игенетики микроорганизмов; доцент кафедры химии и биотехнологии
  • N.G. Muzyka, Institute of Ecology and Genetics of Microorganisms UB RAS
    кандидат биологических наук, старший научный сотрудник лаборатории физиологии и генетики микроорганизмов
  • Z.Y. Samoilova, Institute of Ecology and Genetics of Microorganisms UB RAS
    кандидат биологических наук, старший научный сотрудник лаборатории физиологии и генетики микроорганизмов
  • A.V. Tyulenev, Institute of Ecology and Genetics of Microorganisms UB RAS
    кандидат биологических наук, инженер лаборатории физиологии и генетики микроорганизмов
  • K.V. Bezmaternykh, Institute of Ecology and Genetics of Microorganisms UB RAS
    инженер лаборатории физиологии и генетики микроорганизмов
  • M.A. Peters, Institute of Ecology and Genetics of Microorganisms UB RAS
    аспирант лаборатории физиологии и генетики микроорганизмов
  • V.Y. Ushakov, Institute of Ecology and Genetics of Microorganisms UB RAS; Perm State National Research University
    кандидат биологических наук, ведущий инженер лаборатории физиологии и генетики микроорганизмов; доцент кафедры физиологии растений и микробиологии
  • M.N. Brysova, Institute of Ecology and Genetics of Microorganisms UB RAS
    старший лаборант лаборатории физиологии и генетики микроорганизмов

References

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Published

2017-11-16

Issue

No. 3 (2017)

Section

Research: theory and experiment

How to Cite

Smirnova, G. ., Lepekhina, E. ., Muzyka, N. ., Samoilova, Z. ., Tyulenev, A. ., Bezmaternykh, K. ., Peters, M. ., Ushakov, V. ., & Brysova, M. . (2017). The role of thiol redox systems under a combined action of extreme temperatures and antibiotics in Escherichia coli. Perm Federal Research Centre Journal, 3, 63-67. https://journal.permsc.ru/index.php/pscj/article/view/PSCJ2017n3p10