CHEMICAL AND ENZYMATIC SYNTHESIS OF BIOLOGICALLY ACTIVE COMPOUNDS BASED ON BETULIN

Authors

  • I.B. Ivshina Institute of Ecology and Genetics of Microorganisms UB RAS Perm State National Research University
  • E.V. Tarasova Institute of Ecology and Genetics of Microorganisms UB RAS
  • I.A. Tolmacheva Institute of Technical Chemistry UB RAS
  • V.V. Grishko Institute of Technical Chemistry UB RAS

DOI:

Keywords:

betulin, actinobacteria, Rhodococcus, cell-substrate interactions, biologically active compounds

Abstract

Biotransformation of polycyclic terpenoids by microorganisms is an efficient method for single- stage production of biologically active compounds with high regio- and stereoselectivity. In the previous studies we have reported about the possible directed transformation of betulin by Rhodococcus actinobacteria to produce pharmacologically valuable betulone. In order to increase the efficiency of the process of biotransformation of betulin, this study focuses on the interactions between rhodococci and hydrophobic triterpene betulin, possible ways of betulin transport into bacterial cells, and the localization of enzymes responsible for betulin oxidation. A detailed study on rhodococci and betulin interactions was performed using a combined system of scanning probe microscopy. The effects of betulin on fatty acid composition of rhodococci and their morphometric and morphofunctional characteristics during biotransformation were studied. A general algorithm of the interactions between bacterial cells and betulin was proposed. New triterpene derivatives with a marked antitumor activity were synthesized based on betulone obtained by a biotechnological method.

Supporting Agencies
Исследования поддержаны грантом № 14-04-96017 Российского фонда фундаментальных исследований и Правительства Пермского края.

Author Biographies

  • I.B. Ivshina, Institute of Ecology and Genetics of Microorganisms UB RAS Perm State National Research University
    доктор биологических наук, академик РАН, заведующая лабораторией алканотрофных микроорганизмов; профессор кафедры микробиологии и иммунологии
  • E.V. Tarasova, Institute of Ecology and Genetics of Microorganisms UB RAS
    кандидат биологических наук, научный сотрудник лаборатории алканотрофных микроорганизмов
  • I.A. Tolmacheva, Institute of Technical Chemistry UB RAS
    кандидат химических наук, старший научный сотрудник лаборатории биологически активных соединений
  • V.V. Grishko, Institute of Technical Chemistry UB RAS
    кандидат химических наук, заведующая лабораторией биологически активных соединений

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Published

2017-09-05

Issue

No. 2 (2017)

Section

Research: theory and experiment

How to Cite

Ivshina, I. ., Tarasova, E. ., Tolmacheva, I. ., & Grishko, V. . (2017). CHEMICAL AND ENZYMATIC SYNTHESIS OF BIOLOGICALLY ACTIVE COMPOUNDS BASED ON BETULIN. Perm Federal Research Centre Journal, 2, 46-53. https://journal.permsc.ru/index.php/pscj/article/view/PSCJ2017n2p6