Conugative transfer of bacteriocin genes - new mechanismof antimicrobial action of probiotic preparations

Authors

  • M.V. Kuznetsova Institute of Ecology and Genetics of Microorganisms UB RAS
  • I.L. Maslennikova Institute of Ecology and Genetics of Microorganisms UB RAS
  • D. Žgur-Bertok University of Ljubljana
  • Erjavec M. Starčič University of Ljubljana

DOI:

https://doi.org/10.7242/1998-2097/2017.4.7

Keywords:

Escherichia coli, bacteriocins, colicin ColE7, conjugation, probiotic preparation

Abstract

In connection with the high rate of antibiotic resistance growth of microorganisms, the creation of alternative antimicrobial agents or methods for their delivery is a priority in biology, medicine and veterinary medicine. The results of the design and testing of the ColE7-mediated "kill"-"anti-kill" system based on the probiotic strain Nissle 1917 are presented. The genetically modified strain Escherichia coli ŽP (donor killer) has been made at University of Ljubljana (Slovenia), carrying the colicin gene СolE7 with DNA-ase activity on the conjugative plasmid, as well as the immE7 gene in the chromosome. It provides the cell with the synthesis of an immune protein that binds tightly to the corresponding colicin, inhibiting its activity within the host cell. The system has been tested with the reference E. coli strain in various experimental models: in plankton culture, in a forming and formed biofilm. It has been shown that the antimicrobial action based on the conjugative transfer of bacteriocin genes is possible. Prospects for the use of E. coli ŽP as the basis of a probiotic preparation, which, unlike analogues, will have a high antibacterial activity against enteropathogens due to a new delivery mechanism of colicin, allowing an effective treatment of strains resistant to bacteriocins are identified.

References

  1. Danilevskaa N.V., Subbotin V.V. Effektivnost’ specificeskoj profilaktiki boleznej ptic // 25 let na blago promyslennogo pticevodstva: Materialy ubil. konf. NPP <>. - SPb: AVIVAK, 2015. - S. 107-111.
  2. Nacional’naa koncepcia profilaktiki infekcij, svazannyh s okazaniem medicinskoj pomosi, i informacionnyj material po ee polozeniam / V.I. Pokrovskij, V.G. Akimkin, N.I. Briko, E.B. Brusina, L.P. Zueva, O.V. Kovalisena, V.L. Stasenko, A.V. Tutel’an, I.V. Fel’dblum, V.V. Skarin. - N. Novgorod: izd-vo <>, 2012. - 84 s.
  3. Panteleeva A.A. Geny produkcii mikrocina Escherichia coli S5/98, ih ekspressia i vlianie na antagonisticeskie svojstva rekombinantnyh stammov: avtoref. dis.. kand. biol. nauk, 2006. - 26 s.
  4. Pohilenko V.D., Perelygin V.V. Bakteriociny: ih biologiceskaa rol’ i tendencii primenenia // Elektronnyj naucnyj zurnal <> http://zhurnal.ape.relarn.ru/articles/2011/016.pdf
  5. Cesnokova V.L., Livsic V.A., Sokurenko E.V., Alesin V.V., Kravcov E.G., Dalin M.V., Bykov V.A. Stamm bakterij Escherichia coli m17 fimh::kan/p colap, ispol’zuemyj dla polucenia probioticeskogo preparata // Patent RF No 2144954.
  6. Allen H.K., Trachsel J., Looft T., Casey T.A. Finding alternatives to antibiotics // Ann. N. Acad. Sci. - 2014. - Vol. 1323. - P. 91-100.
  7. Barriere S.L. Clinical, economic and societal impact of antibiotic resistance // Expert Opin. Pharmacother. - 2015. - Vol. 16. - P. 151-153.
  8. Ben Lagha A., Haas B., Gottschalk M., Grenier D. Antimicrobial potential of bacteriocins in poultry and swine production // Vet. Res. - 2017. - Vol. 48. - P. 22.
  9. Budic M., Rijavec M., Petkovsek Z., Zgur-Bertok D. Escherichia coli bacteriocins: antimicrobial efficacy and prevalence among isolates from patients with bacteraemia // PLoS One. - 2011. - Vol. 6(12). - e28769.
  10. Cao Z., Klebba P.E. Mechanisms of colicin binding and transport through outer membrane porins // Biochimie. - 2002. - Vol. 84. - P. 399-412.
  11. Cascales E., Buchanan S.K., Duche D., Kleanthous C., Lloubes R. [et al.] Colicin Biology // Microbiol. Mol. Biol. Rev. - 2007. - Vol. 71. - P. 158-229.
  12. Castanon J.I. History of the use of antibiotic as growth promoters in European poultry feeds // Poult. Sci. - 2007. - Vol. 86(11). - P. 2466-2471.
  13. Cursino L., Smajs D., Smarda J., Nardi R.M., Nicoli J.R., Chartone-Souza E., Nascimento A.M. Exoproducts of the Escherichia coli strain H22 inhibiting some enteric pathogens both in vitro and in vivo // J. Appl. Microbiol. - 2006. - Vol. 100(4). - P. 821-829.
  14. Cursino L., Smarda J., Chartone-Souza E., Nascimento A. Recent updated aspects of colicins of Enterobacteriaceae // Brasil. J. Microbiol. - 2002. - Vol. 33. - P. 185-195.
  15. Dunlap P. Biochemistry and genetics of bacterial bioluminescence // Adv. Biochem. Eng. Biotechnol. - 2014. - Vol. 144. - P. 37-64.
  16. Fernebro J. Fighting bacterial infections-future treatment options // Drug. Resist. Updat. - 2011. - Vol. 14. - P. 125-139.
  17. Filutowicz M., Burgess R., Gamelli R.L., Heinemann J.A., Kurenbach B., Rakowski S.A., Shankar R. Bacterial conjugation-based antimicrobial agents // Plasmid. - 2008. - Vol. 60. - P. 38-44.
  18. Gratia A. Sur un remarquable example d’antagonisme entre deux souches de colibacille // Comput. Rend. Soc. Biol. - 1925. - Vol. 93. - P. 1040-1042.
  19. Grozdanov L., Raasch C., Schulze J., Sonnenborn U., Gottschalk G., Hacker J., Dobrindt U. Analysis of the genome structure of the nonpathogenic probiotic Escherichia coli strain Nissle 1917 // J. Bacteriol. - 2004. - Vol. 186(16). - P. 5432-5441.
  20. Guglielmetti E., Korhonen J.M., Heikkinen J., Morelli L., von Wright A. Transfer of plasmid-mediated resistance to tetracycline in pathogenic bacteria from fish and aquaculture environments // FEMS. Microbiol. Lett. - 2009. - Vol. 293. - P. 28-34.
  21. Karpinski T.M., Szkaradkiewicz A.K. Characteristic of bacteriocines and their application // Pol. J. Microbiol. - 2013. - Vol. 62. - P. 223-235.
  22. Kleanthous C. Swimming against the tide: progress and challenges in our understanding of colicin translocation // Nat. Rev. Microbiol. - 2010. - Vol. 8. - P. 843-848.
  23. Kleanthous C., Walker D. Immunity proteins: enzyme inhibitors that avoid the active site // Trends Biochem. Sci. - 2001. - Vol. 26. - P. 624-631.
    Marincs F. On-line monitoring of growth of Escherichia coli in batch cultures by bioluminescence // Appl. Microbiol. Biotechnol. - 2000. - Vol. 53. - P. 536-541.
  24. Petkovsek Z., Zgur-Bertok D., Starcic Erjavec M. Colicin insensitivity correlates with a higher prevalence of extraintestinal virulence factors among Escherichia coli isolates from skin and soft-tissue infections // J. Med. Microbiol. - 2012. - Vol. 61(6). - P. 762-765.
  25. Schamberger G.P., Phillips R.L., Jacobs J.L., Diez-Gonzalez F. Reduction of Escherichia coli O157:H7 populations in cattle by addition of colicin E7-producing E. coli to feed // Appl. Environ. Microbiol. - 2004. - Vol. 70. - P. 6053-6060.
  26. Smarda J., Smajs D. Colicins - Extracellular lethal proteins of Escherichia coli // Folia Microbiol. (Praha). - 1998. - Vol. 43. - P. 563-582.
    Stahl C.H., Callaway T.R., Lincoln L.M., Lonergan S.M., Genovese K.J. Inhibitory activities of colicins against Escherichia coli strains responsible for postweaning diarrhea and edema disease in swine // Antimicrob. Agents Chemother. - 2004. - Vol. 48. - P. 3119-3121.
    Starcic Erjavec M., Petkovsek Z., Kuznetsova M.V., Maslennikova I.L., Zgur-Bertok D. Strain ZP - the first bacterial conjugation-based "kill"-"anti-kill" antimicrobial system // Plasmid. - 2015. -Vol. 3(82). - R. 28-34.
    Tenover F.C. Development and spread of bacterial resistance to antimicrobial agents: an overview // Clin. Infect. Dis. - 2001. - Vol. 33. - S108-S115.
    Trautner B.W., Hull R.A., Darouiche R.O. Colicins prevent colonization of urinary catheters // J. Antimicrob. Chemother. - 2005. - Vol. 56. - P. 413-415.
    WHO’s first global report on antibiotic resistance reveals serious, worldwide threat to public health. Retrieved 2014-05-02.
    Yang S.-C., Lin C.-H., Sung C.T., Fang J.-Y. Antibacterial activities of bacteriocins: application in foods and pharmaceuticals // Front. Microbiol. - 2014. - Vol. 5. - P. 241.
    Zgur-Bertok D. Regulating colicin synthesis to cope with stress and lethality of colicin production // Biochem. Soc. Trans. - 2012. - Vol. 40 (6). - P. 1507-1511.

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Published

2018-07-25

Issue

No. 4 (2017)

Section

Research: theory and experiment

How to Cite

Kuznetsova, M. ., Maslennikova, I. ., Žgur-Bertok, D. ., & Starčič, E. M. (2018). Conugative transfer of bacteriocin genes - new mechanismof antimicrobial action of probiotic preparations. Perm Federal Research Centre Journal, 4, 45-52. https://doi.org/10.7242/1998-2097/2017.4.7